Highthroughput microscopy, also often referred to as highcontent screening hcs, allows acquisition of systematic data at the singlecell. Highcontent screening for quantitative cell biology. The ics high content screening service boasts two plate imaging platforms, imagexpress ultra, a true point scanning confocal microscope and the imagexpress micro, a fluorescence widefield system with environmental control and drug robot. Automated microscope based high content screening hcs, or hca, hci has gained significant momentum recently due to its ability to study many features simultaneously in complex biology systems. Brochures are available in adobe acrobat pdf format. Highcontent screening hcs is the combination of highcontent analysis and hts bickle, 2010, offering richer data, higher throughput, and increased flexibility usaj et al. High content screening in neurodegenerative diseases protocol. This second edition details some of the recent trends in hcshcahci. The system handles fixed and live cells with equal ease and is therefore the perfect screening platform. Attending this event will provide you with excellent opportunities for networking with attendees from big pharmaceutical and biotech companies, academia and. Phenotypic drug discovery with high content screening. Highcontent screening market global industry analysis. A highcontent screening of anticancer compounds suggests the.
In particular, highcontent screening hcs by imaging cell populations has. High content screening services as an epacertified subcontractor and assay provider for the toxcast screening program, we were recently awarded an exceptional rating for services provided. The portfolio of application modules supports a range of needs from easeofuse through to proprietary assay design. Drug discovery in its early history used to be conducted mainly in a targetagnostic fashion, focusing on qualitative readouts from a living system cells, tissue, or organisms and asking the question, did this entity affect the phenotype. Hcs can be used all along the preclinical drug discovery pipeline, it has the power to identify and validate new drug targets or new lead compounds, to predict in vivo toxicity, and to suggest pathways. Can be used for high throughput screening hts and high content screening hcs, is to help you carry out new drug screening, the emergence of new indications and other professional tools. High content screening hcs technology combining automated. High content screening hcs is a further development of cellbased screening in. Capitalizing this technology, ample cellular information can be extracted from the high content cellular images.
One type of phenotypic screening, imagebased high content screening hcs, has been seen as particularly promising. Here, we aimed at repurposing fdaapproved drugs for nb treatment by performing a highcontent screening of a 349 anticancer. Hot topics to be covered include 3d cell based screening methods, high content screening and data management, the use of model organisms and novel approaches for phenotypic screening. Computer vision for high content screening request pdf. Implementing high content screening hcs into your assay. In the past decade, highcontent screening has become a highly developed approach to obtaining richly descriptive quantita. The report provides separate comprehensive analytics for the us, canada, japan, europe, asiapacific, and rest of world. High content screening hcs, also known as high content analysis, or hca is an approach to cell biology that combines automated imaging and quantitative data analysis in a high throughput format suitable for largescale applications such as drug discovery and systems biology. High content screening hcsbased image analysis is becoming an important and widely used research tool. Sara cherry, university of pennsylvania department of microbiology using stem cellderived neurons in high content screening assays for drug toxicity. High content screening in neurodegenerative diseases.
Pdf a highcontent screening of anticancer compounds. A high content screening of anticancer compounds suggests the multiple tyrosine kinase inhibitor ponatinib for repurposing in neuroblastoma therapy april 2018 molecular cancer therapeutics 177. Screens have been applied to study diverse processes, such as proteinlocalization changes, cancer cell vulnerabilities. High content screening hcs or high content analysis hca also known as cellomics, is a set of analytical techniques that is applied in genetic research and drug discovery processes in order to recognize elements such as peptides, small molecules, or genetic materials such as rnai that have the potential to modify the phenotype of a cell in a certain manner. Development of custom image analysis routines allowed automated compound evaluation to specifically identify induction of cell death in mcts core regions. In this study, an automated, reliable and quantitative cellular image analysis system developed in house has been employed to quantify. However, children with highrisk nb still show high mortality rates prompting for a search of novel therapeutic options. This integrated platform allows for the encapsulation of single cells and reagents in independent aqueous microdroplets 1 pl to 10 nl volumes dispersed in an immiscible carrier oil and enables the digital manipulation of these reactors at. Past, present, and future of high content screening and the field of cellomics d. Using highthroughput rnai screening to identify cellular factors involved in hostpathogen interactions. High content screening hcs is the combination of high content analysis and hts bickle, 2010, offering richer data, higher throughput, and increased flexibility usaj et al.
Lansing taylor summary high content screening hcs was created in 1996 to offer a new platform that could be used to permit relatively highthroughput screening of cells, in which each cell in an array would be analyzed at a sub. High content screening hcs technologies that combine automated fluorescence microscopy with high throughput biotechnology have become powerful systems for studying cell biology and drug screening. Highcontent imaging can be characterized by the combining. Hence high content screening is a type of phenotypic screen conducted in cells involving the analysis. Novel druggable targets have been discovered in neuroblastoma nb, paving the way for more effective treatments. Highcontent screening hcs as a methodological tool has evolved relatively recently, largely driven by the demand for in depth spatial and temporal information from intact cells exposed to a. Imagebased screening is used to measure a variety of phenotypes in cells and whole organisms. High content screening the university of nottingham. To download a copy of the current brochure, please fill out the user profile form below and hit the submit button. Using a combination of established technologies, the cellinsight hcs platforms can help address both cellularlevel intensity and morphological. Opera high content screening system opera high content screening system a new era of cell screening sciences perkinelmer inc. New and updated chapters guide readers through methods that utilize reagents and kits that have been developed to measure cells and. The separation of excitation wavelengths in time and space is achieved by projecting nonoverlapping excitation pinhole patterns onto the sample.
Targetbased high throughput screening hts has recently been critiqued for its relatively poor yield compared to phenotypic screening approaches. The global market is further analyzed by the following segments. To quantify such features one has to move to multidimensional phenotypes termed highcontent screening hcs 4, 1517. Highcontent screening hcs, also known as high content analysis, or hca is an approach to cell biology that combines automated imaging and quantitative data analysis in a high throughput format suitable for largescale applications such as drug discovery and systems biology. High content profiler perkinelmer informatics enterprise. Implementing high content screening hcs into your assay biology. The ease with which data can now be generated has led to a new culture of high throughput science, in which new types of biological questions can be asked and tackled in a systematic and unbiased manner. Using high throughput rnai screening to identify cellular factors involved in hostpathogen interactions. The high content screening includes the software for image capturing, its evaluation and data storage. This approach has been used to address diverse biological questions and identify a plethora of quantitative phenotypes of varying complexity in numerous different model systems. Setting up a 3d high content imaging assay to study.
Screening libraries for high throughputcontent screening. Abstract phenotypic or high content screening hcs is becoming more. In this article, we assess whether hcs is as high content as it can be. High content screening hcs uses microscopic imaging to observe, measure and analyse multiple parameters within cells in a biological assay. High content screening hcs is a drug discovery method which is used to identify chemical substances e. Highcontent screening hcs is a wellestablished approach for the multi parametric analysis of. Here we have shown how a high content assay to study hepatobilary transporter activity can be established in a complex 3d organotypic in vitro liver model system.
The ease with which data can now be generated has led to a new culture of highthroughput science, in which new types of biological questions can be asked and tackled in a systematic and unbiased manner. A powerful approach to systems cell biology and drug discovery, second edition aims to ensure successful results in the further study of this vital field. Hcs is the cellbased quantification of several processes simultaneously, which provides a more detailed representation of the cellular response to various perturbations compared to hts. One upandcoming paradigm shift is to combine the emerging multiple types of. The information content of the typical hcs experiment is much lower than its potential. High content screening hcs was developed by cellomics inc. Droplet microfluidic technology for singlecell high. Not just hardware, but a complete hcs portfolio that includes. Highcontent screening platforms overview thermo fisher. High throughput microscopy, also often referred to as high content screening hcs, allows acquisition of systematic data at the singlecell.
When screening large libraries with 100,000 compounds or more, speed and sensitivity are crucial. The 3d high content screening performed in chp4, imr32, and sknbe2 spheroids resulted in compound lists greatly overlapping among the cell lines tested. Generative adversarial network for single image superresolution in high content screening microscopy images computervision deeplearning imagereconstruction gan imagegeneration convolutionalneuralnetworks perceptuallosses superresolution generativeadversarialnetworks vgg19 srgan microscopyimages high content screening. Interested students must complete the below application for the 2017 student fellowship. High content screening station designed for fully automated image acquisition and data analysis of biological samples. Apr 25, 2017 hot topics to be covered include 3d cell based screening methods, high content screening and data management, the use of model organisms and novel approaches for phenotypic screening. Highcontent screening hcs or highcontent analysis hca also known as cellomics, is a set of analytical techniques that is applied in genetic research and drug discovery processes in order to recognize elements such as peptides, small molecules, or genetic materials such as rnai that have the potential to modify the phenotype of a cell in a certain manner. Structurally diverse, medicinally active, and cell permeable, nmr and hplc validated to ensure high purity. Hcs technology is integrated into all aspects of contemporary drug discovery, including primary compound screening, postprimary screening. Highcontent analysis cellinsight highcontent analysis platforms 22 cellinsight cx7 lzr highcontent analysis platform 24 cellinsight cx5 highcontent screening platform 25 hcs studio cell analysis software 26 store image and database management software 27 cell counting countess ii automated cell counters 28 plate reading microplate readers 30. Image analysis in high content screening request pdf. Hcs can be used all along the preclinical drug discovery pipeline, it has the power to identify and validate new drug targets or new lead compounds, to predict in vivo.
Hcs is also used to discover and optimize new drug candidates. Highcontent screening hcs, which combines automated fluorescence microscopy with quantitative image analysis, allows the acquisition of unbiased multiparametric data at the single cell level. Highcontent screening hcs, also known as highcontent analysis hca or cellomics, is a method that is used in biological research and drug discovery to identify substances such as small molecules, peptides, or rnai that alter the phenotype of a cell in a desired manner. A highcontent screening of anticancer compounds suggests. High content screening hcs, also known as high content analysis hca or cellomics, is a method that is used in biological research and drug discovery to identify substances such as small molecules, peptides, or rnai that alter the phenotype of a cell in a desired manner. High content screening is a combination of modern cell biology, with all its molecular tools, with automated high resolution microscopy and robotic handling. Sara cherry, university of pennsylvania department of microbiology using stem cellderived neurons in highcontent screening assays for drug toxicity.
Authoritative and practical, high content screening and analysisthe ideal format for phenotypic screening. At present, hcs is primarily a fluorescence imaging. Latest developments in high content screening systems. Highcontent screening opera phenix highcontent screening. Finally, manual counting and plating of cells can lead to daytoday. At present, hcs is primarily a fluorescence imaging method. Highcontent screening hcs, also known as highcontent analysis, or hca is an approach to cell biology that combines automated imaging and quantitative data analysis in a highthroughput format suitable for largescale applications such as drug discovery and systems biology. High content screening is a powerful technology that combines automated microscopy typically fluorescence, massive image analysis and data visualization tools. The high throughput and high content screening htshcs core facility specializes in chemical biology as it relates to drug discovery, biological probe discovery, and phenotypic characterization of smallmolecule effects on cellular systems.
Automated microscopy systems allow running these high content assays in a high throughput up to 5,000 10,000 substances per day. We present a dropletbased microfluidic technology that enables highthroughput screening of single mammalian cells. Optics confocal imaging unit pointscan confocal nipkowspinning disc ultra fast laserbased auto focus fully automated exchange of. High content screening hcs as a methodological tool has evolved relatively recently, largely driven by the demand for in depth spatial and temporal information from intact cells exposed to a. High content profiler enables your high content screening experiments to reach their full potential in one single solution powered by tibco spotfire powered by tibco spotfire, high content profiler allows you to transform your high content screening analyses into robust biological conclusions while gaining immediate insights with realtime visual analytics in mere minutes. Based on geography, the global high content screening market is segmented into north america, europe, asia, and the rest of the world row. Hence high content screening is a type of phenotypic screen conducted. Highcontent screening an overview sciencedirect topics.
Given the power of hcs to provide multiparametric readouts, we were surprised to find that roughly 6080% of the. Lansing taylor summary high content screening hcs was created in 1996 to offer a new platform that could be used to permit relatively high throughput screening of cells, in which each cell in an array would be analyzed at a sub. High content screening is an image based screening technique, which measures the physical and biochemical characteristics in the cells and the organisms. Generative adversarial network for single image superresolution in high content screening microscopy images. In addition, compounds that were already in use for nb treatment i.
Combining phenotypic classification with chemical classifica. Highcontent screening hcs on multicellular tumor spheroids mcts. The service also has analysis algorithms from molecular devices metaxpress and a dedicated image analyst. Excitation lasers are placed into two groups so that adjacent laser lines are in different groups. A powerful approach to systems cell biology and phenotypic drug discovery paul a. High content screening hcs on multicellular tumor spheroids mcts. Combined with perturbations such as rna interference, small molecules, and mutations, such screens are a powerful method for gaining systematic insights into biological processes. As an epacertified subcontractor and assay provider for the toxcast screening program, we were recently awarded an exceptional rating for services provided our portfolio includes over 100 primary and stemcell based hcs assays, many of which can be adapted or customized to answer specific biological questions. Our portfolio includes over 100 primary and stemcell based hcs assays, many of which can be adapted or customized to answer specific biological. Live cell imaging, high content screening, cell growth and confluence, cell migration and wound healing, cell cycle protein expression, calcium assays, determining transfection efficiency, behavior of stem cells, cell death assays, spheroid development and behavior, study of lower eukaryotes. Explore our high content screening platforms that are designed to provide exceptional resolution for subcellular detection, automated detection, and phenotyping with intact, fixed, or live cells. A hcsworkflow for t47d mcts generated on 384well agarosecoated multiplates. In the resulting 118 papers 33 150 based on the search above, we then read the relevant portions to identify the main readouts of each highthroughput imagebased experiment.
Paper phenotypic drug discovery with high content screening target based or phenotypic screening. Highcontent screening hcs in cellbased systems uses living cells as tools in biological research to elucidate the workings of normal and diseased cells. Apr 22, 2016 high content screening hcs, which combines automated fluorescence microscopy with quantitative image analysis, allows the acquisition of unbiased multiparametric data at the single cell level. Past, present, and future of high content screening and. High throughput and high content screening core facility. Live cell imaging, high content screening, cell growth and confluence, cell migration and wound healing, cell cycle protein expression, calcium assays, determining transfection efficiency, behavior of stem cells, cell death assays, spheroid development and behavior, study of. This course provides an introductory level overview of the stateoftheart components of hcs together with some showcases of highcontent screens in. Synchrony optics the novel confocal light path of the opera phenix highcontent screening system. This technology is key for drug testing or cell therapy as it allows simultaneous observation, description and classification of multiple cell phenotypes under. To visualize fine structures such as bile canaliculi in 3d, excellent confocal imaging technology is a prerequisite. Past, present, and future of high content screening and the. High content analysis cellinsight high content analysis platforms 22 cellinsight cx7 lzr high content analysis platform 24 cellinsight cx5 high content screening platform 25 hcs studio cell analysis software 26 store image and database management software 27 cell counting countess ii automated cell counters 28 plate reading microplate readers 30. Assay development guidelines for imagebased high content. To quantify such features one has to move to multidimensional phenotypes termed high content screening hcs 4, 1517.
High content screening market global trends, growth. This approach has been used to address diverse biological questions and identify a plethora of quantitative phenotypes of varying complexity in numerous different. In addition to housing the instrumentation and robotics required for traditional hts, the core houses a. Explore our highcontent screening platforms that are designed to provide exceptional resolution for subcellular detection, automated detection, and phenotyping with intact, fixed, or live cells.